Unraveling Pathways and Sources of Selenium Exposure in Sacramento Splittail

This funding is being provided as a cooperative agreement under the Cooperative Ecosystem Studies Unit (CFDA 15.678). The funding will provide for fish otoliths preparation and analysis to evaluate selenium exposure and sources in Sacramento Splittail (Pogonichthys macrolepidotus). Funding authority: Fish and Wildlife Act of 1956, as amended, 16 U.S.C. (742f (a)(4); Fish and Wildlife Coordination Act, as amended, 16 U.S.C. 661.; Fish and Wildlife Improvement Act of 1978, as amended (16 U.S.C. 753), Land and Water Conservation Fund Act of 1965, as amended (16 U.S.C. 4601-4 through 11), Endangered Species Act of 1973 (16 U.S.C. 1531-1543). Unraveling Pathways and Sources of Selenium Exposure in Sacramento Splittail. Otoliths will be prepared using established techniques (Barnett-Johnson et al. 2005, Woodson et al. 2013, Sturrock et al. 2015), cleaned then mounted in Crystalbond resin and polished until the primordia and daily increments are exposed and examined using X-ray fluorescence microscopy to evaluate selenium patterns