The U.S. Army Medical Research Acquisition Activity (USAMRAA) in support of U.S. Army Medical Research Institute of Infectious Diseases (USAMRIID) intends to negotiate solely (as prescribed om FAR 13.106(b)(1)(i) with Pacific Biosciences, 1380 Willow Road, Menlo Park, CA 94025, for the procurement of next-generation sequencing reagents to run the PacBio RSII which is manufactured by Pacific Biosciences. Specifically the government will purchase the following items The following consumables might be required for the Blue Pippin in use at USAMRIID. Several items will be regularly ordered, dependent on available funding, including the DNA template preparation, DNA/Polymerase binding and sequencing kits, DNA control complex, Lambda library complex, SMRT cells, SMRT oil, AMPure PB, MagBead kits, sequencing plate septa, sample plate septa, tube septa, pipet tips and mixing plates . The remaining items could be ordered, dependent on mission requirements. Due to the nature of unforeseeable and emerging occurrences of laboratory studies in USAMRIID and the expectations of support from the Clinical Laboratory, Division of Medicine, additional reagents may be required based on the protocol's specific testing requirements. The following minimal essential characteristics are required: All of the below reagents are required to be licensed for use on the Pacific Biosciences system. PN: 100-171-800 SMRT Cell 8Pac V3 (8 Cells) Individual 8Pac must contain 8 SMRT Cells V3 SMRT Cells must be used in conjunction with the DNA Sequencing Kit for automated processing on the PacBio RS system. One SMRT Cell is consumed per sequencing reaction. PN: 100-229-400 DNA Sequencing Bundle 2.0 (10 Pack) The DNA Sequencing Bundle 2.0 must contain following reagent and disposable supplies for 80 reactions of single molecule real-time sequencing: 10 DNA Sequencing Kits 2.0, 1 SMRT Cell Oil pack, 1 Sequencing Plate Septa pack, 1 Tube Septa pack. PN: 100-216-400 DNA Sequencing Kit 2.0 (8 Rxn) The DNA Sequencing Kit 2.0 must contain a sequencing reagent plate with C2 chemistry and OS Enzyme for single molecule real-time sequencing on the PacBio RS, and must be used in conjunction with SMRT Cell 8Pac(s) PN: 100-254-800 DNA Sequencing Reagent 3.0 The DNA Sequencing Kit 3.0 must contain a sequencing reagent plate with C3 chemistry and OS Enzyme for single molecule real-time sequencing on the PacBio RS, and must be used in conjunction with SMRT Cell 8Pac(s). PN: 100-259-000 DNA Sequencing Bundle 3.0 (10 Pack) The DNA Sequencing Bundle 3.0 must contain reagent and disposable supplies for 80 reactions of single molecule real-time sequencing: 10 DNA Sequencing Reagent plates 3.0, 1 SMRT Cell Oil pack, 1 Sequencing Plate Septa pack, 1 Tube Septa pack. PN: 100-209-300 SMRT(R) Cell Oil SMRT Cell Oil must contain 10 reagent tubes for single molecule, real-time DNA sequencing on the PacBio System to be used in conjunction with the DNA Sequencing Kit. PN: 100-237-900 Sequencing Plate Septa . Septa mat must be able to prevent evaporation of reagents contained in the sequencing plate on the PacBio RS. Each bag must contain 10 septa mats. PN: 100-236-500 DNA/Polymerase Binding Kit P4. The DNA/Polymerase Binding Kit P4 must contain reagent supplies to bind prepared DNA template libraries to the DNA polymerase P4 in preparation for sequencing on the PacBio RS. Reagent quantities must support 24 binding reactions. PN: 100-256-000 DNA/Polymerase Binding Kit P5 The DNA/Polymerase Binding Kit P5 must contain reagent supplies to bind prepared DNA template libraries to the P5-DNA polymerase in preparation for sequencing on the PacBio RS. Reagent quantities must support 24 binding reactions PN: 100-133-600 MagBead Kit The MagBead Kit must contain reagent supplies to capture DNA polymerase and template complex for loading onto the PacBio RS. Kit components must include MagBeads and MagBead Buffer kit. PN: 100-265-900 AMPure PB (5ml) AMPure PB magnetic beads must be specifically formulated for the Pacific Biosciences SMRT sequencing workflow. Reagent quantity must be supplied at 5 mL volume. PN: 000-882-901 Sample Plate Septa 96-well PCR plate septa must prevent evaporation of the sample, loaded on the PacBio RS. Each case must contain 50 plate septa. PN:100-259-100 SMRTbell Template Prep Kit 1.0 The SMRTbell Template Prep Kit 1.0 must contain reagent supplies to perform SMRTbell library preparations of primerannealed SMRTbell libraries for insert sizes ranging from 500 bp to over 20 kb. Reagent quantities must support 10 library preparations. PN:100-286-300SMRTbell HT Template Prep Kit 1.0 The SMRTbell HT Template Prep Kit 1.0 must contain reagent supplies to perform 96 automated SMRTbell library preparations of primer-annealed SMRTbell libraries for insert sizes ranging from 500 bp to over 20 kb. Reagents must come in 4 x 24 reaction sets. PN: 100-245-100 DNA Control Complex P4 (250 bp- greater than 3Kb) The DNA Control Complex P4 (250bp less than 3Kb) must contain spike in template bound to DNA Polymerase P4 for support of insert sizes from 250 bp to less than 3Kb. Reagent quantities must provide spike in controls for a minimum of 24 samples. PN: 100-245-200 DNA Control Complex P4 (3kb-10kb) The DNA Control Complex P4 (3Kb - 10 Kb) must contain spike in template bound to DNA Polymerase P4 for support of insert sizes equal or greater than 3Kb. Reagent quantities must provide spike in controls for a minimum of 24 samples. PN: 100-245-300 Plasmidbell Complex P4 (11kb) The Plasmidbell Complex P4 (11Kb) must contain a fixed length DNA template of 11Kb bound to DNA Polymerase P4. PN: 100-258-900 Plasmidbell Complex P5 (11kb) The Lambda Lib Complex P5 (2Kb) must contain Lambda library DNA template sheared to a peak size 2Kb bound to Polymerase P5. PN: 100-245-000 Lambda Lib Complex P4 (2kb) The Lambda Lib Complex P4 (2Kb) must contain Lambda library DNA template sheared to a peak size 2Kb bound to Polymerase P4. PN: 001-292-541 Tube Septa 1 bag of tube septa must be able to control evaporation of OS enzyme and SMRT Oil on the PacBio RS. Each bag must contain 20 tube septa. PN:000-448-888 Sample Plate 96 well PCR plates must be approved for sample placement on the PacBio RS. Each case must contain 50 plates. PN: 100-243-500 Mixing Plate (40 pack) 384 well deep well plates must be approved for reagent and sample mixing on the PacBio RS. Each case must contains 40 plates. PN: 000-947-365 Mixing Plate (160 pack) 384 well deep well plates must be approved for reagent and sample mixing on the PacBio RS. Each case must contains 160 plates PN: 001-252-068 Pipet Tips (50 pack) Sterile filter tips must be approved for robotic liquid handling on the PacBio RS. Each case must contain 50 racks of 384 tips each. PN:100-154-200 Pipet Tips (10 pack) Sterile filter tips must be approved for robotic liquid handling on the PacBio RS. Each case must contain 10 racks of 384 tips each. The North American Industry Classification System (NAICS) for this requirement is 325414 and the size standard is 500 employees. This announcement constitutes the only notice by the government; a written solicitation will not be issued. All sources that believe they are eligible to provide these items must respond in writing. Responses must be supported with clear and convincing evidence to clearly articulate the ability to provide the items outlined above. A request for documentation will not be considered an affirmative response. This Special Notice of Intent is not a request for competitive quotes. Information received will be considered solely for the purpose of determining whether a competitive procurement is in the best interest of the Government. If no written responses are received by the date listed below, which reflects fifteen (15) days after the publication of this notice, the purchase will be awarded without further remark. All questions and responses concerning this notice shall be emailed to Ms. Jennifer Hayden at
[email protected]. Interested concerns must identify their capability in writing to the above e-mail address or by mail to the address listed below no later than 3:00 pm EST, Thursday, June 18, 2014.