Oligo and Gene Production for CVV Develop

The Centers for Disease Control and Prevention (CDC), the Vaccine Preparedness Team of the Virology, from the Virology Surveillance and Diagnosis Branch within the Influenza Division of the CDC intends to competitively procure through full and open competition of recombinant antibodies. The overall objective is to provide the Influenza Division with custom genetic products for the development of CVVs. The Vaccine Preparedness Team of the Virology, from the Virology Surveillance and Diagnosis Branch within the Influenza Division of the CDC shall be the principal scientific and technical party representing the Government’s needs, working with an indetermined Contractor from selected Offerors. The CDC will provide specific sequences, digitally, for each requested genetic product of the appropriate size. Requirements: Task 1: Double-stranded DNA gene fragments of 300-1,500bp To a total maximum length of 105,000bp across all fragments Error rate of less than 1 bp in 5,000bp Minimum yield 200ng Resuspended in nuclease-free water to a concentration of 10 ng/µl Task 2: Double-stranded DNA gene fragments of 1,751-2,000bp To a total maximum of 125 preparations Error rate of less than 1 bp in 5,000bp Minimum yield 1,000ng To be delivered dry, as lyophilized powder Task 3: Double-stranded DNA gene fragments of 2,001-2,250bp To a total maximum of 125 preparations Error rate of less than 1 bp in 5,000bp Minimum yield 1,000ng To be delivered dry, as lyophilized powder Task 4: Single-stranded oligonucleotides ordered in 96-well plate format, 15-60nt in length To a total maximum length of 195,500 nucleotides across all oligos. Concentration to be normalized across plate to 2-10nmol per well presuming sufficient yield, and at full syntheses yield for any oligo with insufficient yield to reach 2nmol in concentration. Any such low-yield oligos must be identified on documentation accompanying the plate in delivery Task 5: Single-stranded oligonucleotides, delivered in individual tubes 15-45nt in length To a total maximum length of 90,000 nucleotides across all oligos. Minimum yield 8ng To be delivered dry or normalized to a concentration of 100 μM. The Government will release the solicitation, including any subsequent amendments on the SAM.gov website. The Government anticipates the solicitation will be available approximately 5 days from the date of release of this notice, with an anticipated closing date/proposal due date of 10 days from the date of release of solicitation. No telephone or written requests for the solicitation will be accepted. After solicitation release, vendors should regularly access the website to access any updates. Attachments: 1. SOW