Pacbio Sequencing of 8 citrus (Pomelo) tissue samples

This award is for a Genomics Project of Pacbio Sequencing of 8 citrus (Pomelo) tissue samples that must run together as a batch of 8. Receive 8 tissue samples and extract HMW DNA suitable for Pacbio sequencing a- Must receive correct amount of tissue and quality of tissue for HMW DNA; b- Extractions performed using modified CTAB; QC data available c- cost for shipping is the responsibility of the customer Produce Pacbio HiFi sequence libraries a- Construct one HiFi library from each DNA sample b- HiFi library, size selected 15-25kb c- Amnt of library correct for Number of cells to run Sequence libraries on Pacbio Sequel II; FOR EACH SAMPLE: a- Sequencing Chemistry used is SeqII v2.0; SMRT cells are 8M v1 b- Sequence 1 cell HiFi CCS (30hr) c- Each CCS cell is expected to produce 16-24Gb of QV20+ CCS data. No guarantee on yield. Run CCS analysis on all raw data; summarize data and deliver via secure FTP a- CCS analysis run on each HiFi CCS cell raw data; b- No additional bioinformatics. No Assembly c- AGI QC data, monitor progress, summarize outputs and stats. Deliver CCS data via secure FTP (or cloud). TOTAL COST for 8 sample data sets (from 8 cells sequenced) Includes all materials, supplies, labor, and fees