Tetrad 2 Thermal Cycler

19181AM002 This is a notice of intent to go Sol Source in accordance with FAR 6.302-1 for the following items: One, Tetrad 2 Thermal Cycler Chassis. Four bay thermal cycler chassis, does not include Alpha unit reaction modules (requires 4). Four, 96-Well Alpha Unit with Hot Bonnet Heated Lid. 96-well reaction module for DNA Engine Thermal Cyclers, holds one 96 -well plate or 96 x .02 ml tubes, includes sample consumables. Four, 48/48 Dual Alpha Unit with two Heated Lids. Reaction module for DNA Engine thermal cycles, includes 2 independent blocks, each holds 48 x 0.2 ml tubes, also includes sample consumables. Vendor: Bio-Rad Laboratories, Inc.2000 Alfred Nobel Dr.Hercules, CA 94547-1804 Central to the work of the Genetics Section are the procedures, instruments, and reagents used for DNA sequence analysis and STR fragment analysis. The use of validated protocols and instruments and the ability to maintain consistency between procedures is crucial to forensic genetic analyses. The DNA Engine Tetrad Thermal Cycler with 4 x 96-well independent "hot-bonnet" heated lids offered by Bio-Rad is a replacement for an existing DNA Engine Tetrad Thermal Cycler. The 4x Dual-48 "hot bonnet" heads are interchangeable only with the Tetrad 2 base unit. These heads, however can be interchanged between an existing Tetrad 2 in use in the Genetics Laboratory, as well as with a new Tetrad 2. The acquisition of these multi-use heads will help increase efficiency of PCR use for small volume casework. PCR procedures and protocols used in forensic casework have been validated for use on a peltier thermal cycler. Purchase of the Bio-Rad DNA Engine Tetrad Thermal Cycler will allow the genetics section to maintain workflow in support of the FWS/OLE mission without spending additional time and effort to validate PCR protocols on a machine with different thermal parameters. Bio-Rad is the original manufacturer and sole supplier of the DNA Engine Tetrad Thermal Cycler. The Genetics section requires an instrument that will provide an interchangeable platform with an existing DNA Engine Thermal Cycler. The thermal cycler must be capable of independent programming of 4 96-well heads with heated lids, and must e able to accommodate dual 48-well heads. The unit must have an integrated power supply with power supply protection in which programs resume execution upon power-up if the power fails. The unit must include a graphical gradient calculator for gradient temperature protocols and must allow a temperature range of 0 degrees C - 100 degrees C. Thermal accuracy of the unit must be NIST traceable, and within 0.3 degrees C within 30 seconds of arrival at 90 degrees C; and a thermal homogeneity of 0.4 degrees C within 30 sec of arrival at 90 degrees C.