ENVISION XCITE PLATE READER

DESCRIPTION : The National Institutes of Health - NICHD intends to procure the services of a contractor to provide a EnVision Xcite Plate Reader system with software and related components as stated below (SOW). Envision 2105 Xcite configuration will contain a single PMT Unit with Fluorescence Intensity (top and bottom), Absorbance, Standard Luminescence, Fluorescence Polarization, Time-Resolved Fluorescence/FRET. The configured Envision 2105 Multimodal Plate Reader will also include EnVision Manager Software, Win-10 Desktop Computer and 17'' TFT Flat Screen Monitor. The configuration additionally includes our Ultra- Sensitive Luminescence and a Temperature Control Module Optics included in the requested configuration include those for GFP and mCherry assay reads, as well as filters to read absorbance at 420 nm, 550 nm, 595 nm and 600 nm. Additional Envision Optics can be quoted that include various Mirror Modules, Excitation filters, and Emission filters that will allow your Envision 2105 to operate in filter-based mode for the utmost flexibility and sensitivity. Included in your purchase of the Envision Multimodal Plate Reader will also include installation , training , local Sales and Application Support as well as a one year all-inclusive warranty . All servicing and support of the Envision 2105 will be performed on-site to minimize any experimental delays. Unique Features of the PerkinElmer Envision Multi-Label Plate Reader: The Reader must include or be field upgradeable with the following Detection Technologies: o AlphaScreen/AlphaLISA Technology Ultra-Sensitive Luminescence Standard Luminescence Fluorescence Intensity Top and Bottom Time-Resolved Fluorescence Time-Resolved FRET Absorbance (UV & Visible) Fluorescence Polarization Filter-based and Quad Monochromator-based Absorbance and Fluorescence Intensity tunable in 0.1nm increments Temperature Control from 2 degrees above ambient to 55 degrees C. Alpha Technology must use Laser Excitation at the standard 680nm Laser. This yields a significant increase in sensitivity and speed of detection and may be critical for assays which require lowered limit of detection samples. ▪ Alpha Technology: System must be capable of Alpha detection at elevated temperatures from 2 degrees C above ambient to 55 degrees C. ▪ Alpha Technology: Two separate options are available; Alpha HTS "Turbo" Option for high throughput and Alpha Standard Option for low-medium throughput. They both function with single emission reading and are therefore suitable for both single and dual detector models. The typical measurement times when measuring Alpha on a 96-well plate are 30 seconds / plate with Alpha HTS Option and 68 seconds / plate with Alpha Standard Option. Multiplexing is only possible on the Standard Option. Multiplexing can be performed on the dual detector model in parallel, on the single detector subsequently, resulting in ~2 times slower reading time. ▪ The Reader must include or be field upgradeable with the following Additional Hardware: ▪ Time-resolved Fluorescence and TR-FRET Laser with focused Excitation at 337nm ▪ Quad Monochromator Setup to combine the sensitivity of a filter-based reader with the wavelength scanning and versatility of a monochromator-based reader. For Fluorescence Intensity, the 2105 Model will contain 2 Excitation Monochromators and 2 Emission Monochromators. ▪ Ultra-sensitive Luminescence with dedicated PMT ▪ Robotic Microplate Stackers with choice of 20 or 50-plate Magazines Dedicated PMT Detector for Ultra-Sensitive Luminescence detection with the PMT situated in close proximity to the sample microplate. This yields a significant increase in sensitivity and speed of detection, and may be critical for assays which require detection of low-expressing samples. o Ultra-Sensitive Luminescence is 30X more sensitive than standard luminometers ▪ The System must be upgradeable with dual PMT Detectors for Fluorescence Detection/Time-resolved Fluorescence/TR-FRET, enabling fast detection of Dual-emission assays such as FRET and BRET. ▪ Optimization Wizard to automatically determine optimal settings for the following parameters. This will greatly improve the performance of the reader and accelerate assay development: o Microplate Dimensions o PMT Detector Sensitivity o Excitation Lamp Power o Crosstalk Correction for Luminescence Detection o Minimal number of flashes for targeted Z-Prime value o Flat-field Correction o Read Height ▪ Use of Application-specific Dichroic Mirror Modules increases Sensitivity of TRF and TR-FRET assays such as DELFIA and LANCE Modules. ▪ Up to four optimized Dichroic Mirror modules can be installed at once. ▪ Up to eight (8) excitation/absorbance filters and eight (8) emission filters can be installed at one time. This modular design allows for optimized optics for specific applications. ▪ The system must be able to identify all installed Filters and Optical Modules via Barcode detection as this will help prevent user errors in a multi-user environment. ▪ Ability to accommodate 6, 24, 96, 384, 1536, and 3456-well Microplates ▪ The reader must be equipped with Well-Area Scanning ▪ The reader must be equipped with the ability to read kinetic assays ▪ The reader must be able to control temperature of the sample Microplate holder from ambient to 55 degrees C in 0.5 Celsius increments. ▪ EnVision 2105 is now capable to communicate with external 64 bit automation applications for robotic interfaces. ▪ Envision is the only plate reader that has a dedicated field-based team to support the PerkinElmer reagents including Luminescence, Alpha, LANCE, and DELFIA Technologies. General Specifications of the Envision Multi-Label Plate Reader Light source: ** Xenon flash lamp (default for non TRF-laser Measurements) Plate format 1- to 3456-well Temperature control ▪ Temperature range From 2 °C above ambient up to 55 °C (0.5 °C increments) Shaking Linear, orbital, dual orbital Computer Specifications: Desktop Computer Microsoft® Windows® 10 Operating System Connection via CAN-card This proposed procurement is for supplies for which the government intends to solicit and evaluate quotes based on our interpretation of the description. Interested persons may identify their interest and capability to respond to the requirement or submit quotations In order to comply with the requirements in this Solicitation, the responses should be based on our interpretation of the general requirements provided. HOW TO RESPOND: In order to compete for this project, interested parties must be registered in the Central Contracting Registration and submit a price quotation by 10am, ET, July 3rd 2020. Offerors should submit one copy of the quote to MOE FILALI via email. Electronic transmissions to [email protected] are preferable but must be timely. It is the responsibility of the Offeror to ensure the proposal is received by the date and time shown above. The following Federal Acquisition Regulations clauses are incorporated by reference: FAR 52.212-3, Offeror Representations and Certifications-Commercial Items (Mar 2005), 52.212-4 (Sept 2005), 52.212-5, Contract Terms and Conditions Required to Implement Statutes of Executive Orders-Commercial Items (Aug 2006). (6)(i) 52.222-26, Equal Opportunity (Apr 2002) (E.O. 11246). (18) 52.222-35, Equal Opportunity for Special Disabled Veterans, Veterans of the Vietnam Era, and Other Eligible Veterans (Dec 2001) (38 U.S.C. 4212). (19) 52.222-36, Affirmative Action for Workers with Disabilities (Jun 1998) (29 U.S.C. 793). (20) 52.222-37, Employment Reports on Special Disabled Veterans, Veterans of the Vietnam Era, and Other Eligible Veterans (Dec 2001) (38 U.S.C. 4212). (31) 52.232-33, Payment by Electronic Funds Transfer-Central Contractor Registration (Oct 2003) (31 U.S.C. 3332). Primary Point of Contact: MOE FILALI Contracting Office Address: NIH/NICHD Office of Acquisitions 6710B Rockledge Drive, Room 1145D Bethesda, MD 20817 (w) 301.827 7737 [email protected]